Jie Lang, Huibin Yang, Haibo Yu*
State Key Laboratory of the Discovery and Development of Novel Pesticides, Shenyang Sinochem Agrochemicals R&D Co., Ltd., Shenyang 110021, Liaoning, China.
*Corresponding author: Haibo Yu
Abstract
This paper established a rapid, simple method for determination of absolute content of cyetpyrafen by qNMR. 1H NMR spectra was acquired with deuterodimethyl sulfoxide (DMSO-D6) as the solvent and hydroquinone as internal reference standard under the conditions of temperature 25℃, pulse angle 90°, pulses width 8.0 μs, relaxation delay 30 s, and scans 8. The proton peaks of cyetpyrafen (δ=6.35) and hydroquinone (δ=6.55) were taken as quantitative peaks. The peak area ratio y (As/Ar) and concentration ratio x (Cs/Cr) were linearly regressed, and the correlation coefficient was 0.9999. The RSD value of repeatability test was 0.80%, and the RSD value of stability test was 0.54%. The content of cyetpyrafen was determined as 99.8%. The result shows that qNMR can be used for quantitative determination of cyetpyrafen without reference standard, which is rapid, accurate and simple.
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How to cite this paper
Determination of Absolute Content of Cyetpyrafen by qNMR Analysis
How to cite this paper: Jie Lang, Huibin Yang, Haibo Yu. (2022) Determination of Absolute Content of Cyetpyrafen by qNMR Analysis. International Journal of Food Science and Agriculture, 6(2), 149-153.
DOI: http://dx.doi.org/10.26855/ijfsa.2022.06.003