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International Journal of Neuroscience and Psychiatry

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ArticleOpen Access http://dx.doi.org/10.26855/ijnp.2025.12.001

Experimental Study on the Anti-inflammatory Effect of Genistein on PC12 Nerve Cells

Ying Zhou*, Ting Xiong, Ying Chen, Haigang Li

Changsha Medical College school of pharmacy, Changsha 410219, Hunan, China.

*Corresponding author: Ying Zhou

Published: November 27, 2025

Abstract

Objective To investigate the effect of genistein on PC12 cell inflammatory model induced by hydrogen peroxide and its anti-inflammatory mechanism. Methods 1. Select the suitable concentration of hydrogen peroxide for modeling to construct PC12 cell inflammation model. 2. The effects of different concentrations of genistein on cell survival were determined. 3. MTT assay was used to observe the protective effect of genistein on PC12 cells with H2O2 induced inflammation. 4. The expression of NgR mRNA in PC12 cells of each group was detected by real-time PCR. Result 1. Genistein concentration 100 μ There is basically no cytotoxicity below M, and genistein pretreatment has a protective effect. Compared with the model group, it significantly improves the cell survival rate of PC12 inflammatory model. 2. Genistein significantly decreased the expression of NgR gene induced by H2O2 damage. Conclusion Genistein can inhibit the inflammation of PC12 neurons by down regulating the level of NgR through Nogo/NgR signaling pathway.

Keyword

Genistein; Neuroinflammation; Anti inflammation; NgR

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Copyright

© 2025 by the author(s).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND) license, which permits non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited and is not modified or adapted.
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How to cite this paper

Experimental Study on the Anti-inflammatory Effect of Genistein on PC12 Nerve Cells

How to cite this paper: Ying Zhou, Ting Xiong, Ying Chen, Haigang Li. (2025). Experimental Study on the Anti-inflammatory Effect of Genistein on PC12 Nerve Cells. International Journal of Neuroscience and Psychiatry1(1), 15-18.

DOI: http://dx.doi.org/10.26855/ijnp.2025.12.001