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Health and Prevention Journal

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ArticleOpen Access http://dx.doi.org/10.26855/hpj.2025.12.004

Evaluation of Quantum Dots Nanobeads Based Lateral Flow Immunoassay for Interluekin-6 Detection in Human Blood for Cervical Inflammation Monitoring

Qianyu Liu

Beijing World Youth Academy, No. 8, Beiyuan, Laiguangying West Road, Chaoyang District, Beijing 100012, China.

*Corresponding author: Qianyu Liu

Published: November 14,2025

Abstract

Sensitive detection of IL-6 in menstrual blood at home is helpful for monitoring and early diagnosis of cervical health. However, traditional gold nanoparticles based lateral flow immunoaasy is not sensitive enough for IL-6 detection in menstrual blood. Quantum dots nanobeads (QDNB) and super-quantum dots nanobeads (SQDNB) were used as labels to prepare e lateral flow immunoassay for IL-6 detection. The concentrations of fluorescent conjugates were optimized, and the analytical performance of the developed assay were evaluated. The optimized dilutions of fluorescent conjugates are 1:200 and 1:100 for QDNB and SQDNB conjugates. The limits of detection of lateral flow immunoassay used QDNB and SQDNB as labels are 0.14 and 0.16pg/mL, respectively. CV for SQDNB at 3.9 pg/mL and 62.5 pg/mL are 18.0% and 20.2% respectively. This sensitive lateral flow assay is potential for self-test of IL-6 in menstrual blood at home.

Keywords

Interleukin-6; cervical health; lateral flow immunoassay; quantum dot nano-beads, antibody

References

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How to cite this paper

Evaluation of Quantum Dots Nanobeads Based Lateral Flow Immunoassay for Interluekin-6 Detection in Human Blood for Cervical Inflammation Monitoring

How to cite this paper: Qianyu Liu. (2025) Evaluation of Quantum Dots Nanobeads Based Lateral Flow Immunoassay for Interluekin-6 Detection in Human Blood for Cervical Inflammation Monitoring. Health and Prevention Journal2(1), 49-53.

DOI: http://dx.doi.org/10.26855/hpj.2025.12.004