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International Journal of Food Science and Agriculture

ISSN Print: 2578-3467 Downloads: 452789 Total View: 4770440
Frequency: quarterly ISSN Online: 2578-3475 CODEN: IJFSJ3
Email: ijfsa@hillpublisher.com
ArticleOpen Access http://dx.doi.org/10.26855/ijfsa.2025.03.001

Assessing the Nutritional Value and Suitability of Processed Rubber (Hevea Brasiliensis) Seed Meal for Rabbit Feed: A Focus on Anti-nutrient Degradation and Processing Methods

Obanla R. Oyinlola1,*, Imen Bousrih2, Ojewumi E. Modupe3, Farouk U. Mohammed4, Akerele E. Mojoyinoluwa5

1Engineering Department, Wake Forest University, Winston-Salem, NC 27109, USA.

2Ralph E. Martin Department of Chemical Engineering, University of Arkansas, Fayetteville, AR 72701, USA.

3Department of Civil & Environmental Engineering, FAMU-FSU College of Engineering, Tallahassee, FL 32310, USA.

4End Use Laboratory, Rubber Research Institute of Nigeria, Benin City 300001, Edo State, Nigeria.

5Department of Chemical Engineering, Covenant University, Ota 112104, Ogun State, Nigeria.

*Corresponding author: Obanla R. Oyinlola

Published: March 21,2025

Abstract

This study investigated the impact of processing methods on the nutritional value of rubber seeds. Two pretreatment techniques were explored to reduce anti-nutrients in the raw seeds: oven-drying the rubber seed meal for 30 to 180 minutes and soaking in an aqueous medium. The processed seeds were then collected and chemically analyzed, with samples taken from each treatment group. The proximate analysis of soaked rubber seed meal (SRSM) revealed the following composition: 2.80% moisture, 4.67% total ash, 18.70% crude protein, 8.34% crude fiber, 31.26% ether extract, and 34.23% carbohydrates. In contrast, oven-dried rubber seed meal (ODRSM) at 300°C for 30 minutes exhibited the highest values for moisture (3.92%), total ash (4.08%), crude protein (25.00%), crude fiber (8.16%), and ether extract (32.68%). Among the amino acids, glutamic acid, arginine, and cystine were the most abundant. Interestingly, oven-drying at 180 minutes proved most effective at removing anti-nutrients, with significant reductions in cyanide (6.53mg/g), tannins (0.051mg/g), saponins (0.1mg/g), and phenols (0.210mg/g) compared to soaking treatment. Based on these findings, the researchers recommend using rubber seeds heated to 300°C for 180 minutes as animal feed. This method results in low ash content, good protein levels, and a rich profile of macro and micronutrients. Overall, oven-drying appears to be a superior method for improving the nutritional value of rubber seed meal.

Keywords

Feed; pretreatment; proximate analysis; oven-drying; rubber seed; nutritive value; livestock

References

[1] Zhu Y, Xu J, Li Q, Mortimer PE. Investigation of rubber seed yield in Xishuangbanna and estimation of rubber seed oil-based biodiesel potential in Southeast Asia. Energy. 2014;69(C):837-42. https://doi.org/10.1016/j.energy.2014.03.079

[2] Yusup S, Khan M. Basic properties of crude rubber seed oil and crude palm oil blend as a potential feedstock for biodiesel production with enhanced cold flow characteristics. Biomass Bioenergy. 2010;34(10):1523-6.

https://doi.org/10.1016/j.biombioe.2010.03.022

[3] Eka HD, Tajul Aris Y, Wan Nadiah WA. Potential use of Malaysian rubber (Hevea brasiliensis) seed as food, feed and biofuel. Int Food Res J. 2010;17:527-34.

[4] Iyayi EA, Fayoyin FK. Effect of feeding cassava fruit coat meal on the nutrient digestibility and performance of broilers. Livest Res Rural Dev. 2005;17(1).

[5] Udo MD, Ekpo U, Ahamefule FO. Effects of processing on the nutrient composition of rubber seed meal. J Saudi Soc Agric Sci. 2018;17(3):297-301. https://doi.org/10.1016/j.jssas.2016.06.001

[6] Aguihe PC, Kehinde AS, Ospina-Rojas CI, Murakami AE. Evaluation of processing methods of rubber (Hevea brasiliensis) seed meal for use as a feed ingredient for broiler chickens. J Poult Res. 2017;14(1):20-7. Print ISSN: 1302-3209. Online ISSN: 2147-9003.

[7] Abdulrashid M, Agwunobi LN. Taro Cocoyam (Colocasia esculenta) meal as feed ingredient in poultry. Pak J Nutr. 2009;8:668-73. https://doi.org/10.3923/pjn.2009.668.673

[8] Tuleun CD, Igba F. Growth and carcass characteristics of broiler chickens fed water soaked and cooked velvet bean (Mucuna utilis) meal. Afr J Biotechnol. 2008;7(15):2676-81.

[9] Abdullah BM, Salimon J, Yousif E, Salih N. Occurrence of cyanogenic glycoside and cyanide in the Malaysian rubber seed oil. J Assoc Arab Univ Basic Appl Sci. 2013;14:83-6. https://doi.org/10.1016/j.jaubas.2012.11.004

[10] Nazok A, Rezaei M, Sayyahzadeh H. Effect of different levels of dried citrus pulp on performance, egg quality, and blood parameters of laying hens in early phase of production. Trop Anim Health Prod. 2010;42:737-42. http://dx.doi.org/10.1007/s11250-009-9481-x

[11] Kinh LV, Kopinski JS, Burren B, Ninh PH, Trung VN. Processing rubber seed into a useful pig feed in Vietnam. In: Proceedings of the XIIth Asian-Australasian Animal Production Congress; 2006 Sep 18-22; Busan, Korea.

[12] Okafor PN, Anyanwu NO. Enzymatic and oven-drying method of processing rubber seeds for animal feed and the evaluation of the toxicity of such feed in rats. Asian J Biochem. 2006;1(1):125-30. https://doi.org/10.3923/ajb.2006.125.130

[13] Jonathan M, Keta JN, Singh D. Identification and evaluation of proximate and antinutritional profile of some underutilized mushrooms in Yauri Local Government Area, Kebbi State, Nigeria. J Adv Educ Sci. 2022;2(4):61-6. DOI: 10.47872/2583-2360/2.4.61-66

[14] Alabi RO. Mycology and Nigerian culture: past, present and future. In: Proceedings of the 1st Conference on African Mycology; 2005; Mauritius. p. 43-52.

[15] AOAC. Official methods of analysis of the association of official analytical chemists. 20th ed. Washington, DC: AOAC; 2016.

[16] AOAC. Official methods of analysis of the association of official analytical chemists. 17th ed. Washington, DC: AOAC; 2000.

[17] Khanna AS. High temperature oxidation. In: Kutz M, editor. Handbook of Environmental Degradation of Materials. 2nd ed. William Andrew Publishing; 2012. p. 127-94. https://doi.org/10.1016/B978-1-4377-3455-3.00005-5

[18] AOAC. Official Methods of Analysis. 15th ed. Washington, DC: Association of Official Analytical Chemists; 1990. p. 200-10.

[19] Sáez-Plaza P, Michałowski T, Navas MJ, Asuero AG, Wybraniec S. An Overview of the Kjeldahl Method of Nitrogen Determination. Part I. Early History, Chemistry of the Procedure, and Titrimetric Finish. Crit Rev Anal Chem. 2013;43(4):178-223. https://doi.org/10.1080/10408347.2012.751786

[20] Hernández-López BA, Sánchez Félix DA, Zuñiga Sierra Z, García Bravo I, Dinkova TD, Avila-Alejandre AX. Quantification of Reducing Sugars Based on the Qualitative Technique of Benedict. ACS Omega. 2020;5(50):32403-10. DOI: 10.1021/acsomega.0c04467

[21] Bajaj KL, Devsharma AK. A colorimetric method for the determination of tannins in tea. Mikrochim Acta. 1977;68:249-53. https://doi.org/10.1007/BF01196209

[22] Makkar HPS. Measurement of Total Phenolics and Tannins Using Folin-Ciocalteu Method. In: Quantification of Tannins in Tree and Shrub Foliage. Dordrecht: Springer; 2003. https://doi.org/10.1007/978-94-017-0273-7_3

[23] Henríquez CA, Almonacid SF, Chiffelle Í, Valenzuela T, Araya MA, Cabezas L, et al. Determination of antioxidant capacity, total phenolic content and mineral composition of different fruit tissue of five apple cultivars grown in Chile. Chil J Agric Res. 2010;70:523-36. DOI:10.4067/S0718-58392010000400001

[24] Ukhun ME, Uwatse V. Nutritional evaluation of selected Nigerian rubber seed products- A chemical approach. Plant Foods Hum Nutr. 1988;38:309-18. https://doi.org/10.1007/BF01091728

[25] Madubuike FN, Ekenyem BU, Obih TKO. Performance and cost evaluation of substituting rubber seed cake for groundnut cake in diets of growing pigs. Pak J Nutr. 2006;5(1):59-61. https://doi.org/10.3923/pjn.2006.59.61

[26] Oyekunle JAO, Omode AA. Chemical Composition and Fatty Acid Profile of the Lipid Fractions of Selected Nigerian Indigenous Oilseeds. Int J Food Prop. 2008;11(2):273-81. https://doi.org/10.1080/10942910701302598

[27] Onwurah INE, Otitoju O, Njoku OU, Okafor FC, Obidiegwu C. Nutritional value of rubber seed flour for poultry feedstock, using adult insect Tribolium castaneum as a model. Arch Appl Sci Res. 2010;2(5):422-8.

[28] Mmereole FUC. The Effects of Replacing Groundnut Cake with Rubber Seed Meal on the Haematological and Serological Indices of Broilers. Int J Poult Sci. 2008;7(6):622-4.

[29] Ly J, Ty C, Phiny C. Evaluation of nutrients of rubber seed meal in Mong Cai pigs. Livest Res Rural Dev. 2001;13:Article #19. Available from: http://www.lrrd.org/lrrd13/2/ly132.htm

[30] Chanjula P, Wanapat M, Wachirapakorn C, Uriyapongson S, Rowlinson P. Ruminal degradability of tropical feeds and their potential use in ruminant diets. Anim Biosci. 2003;16(2):211-6. https://doi.org/10.5713/ajas.2003.211

[31] Udedibie ABI, Carlini CR. Relative effect of dry and moist heat treatments on haemagglutinin and antitryptic activities of selected legume grains. Niger J Poult Sci. 2000;1(1):81-7.

[32] Onu PN, Okongwu SN. Performance characteristics and nutrient utilization of starter broilers fed raw and processed pigeon pea (Cajanus cajan) seed meal. Int J Poult Sci. 2006;5(7):693-7. https://doi.org/10.3923/ijps.2006.693.697

[33] Hossain ME, Karim MH, Alam S, Nath SK. Nutritive value of rubber seed (Hevea brasiliensis). Online J Anim Feed Res. 2015;5(1):18-21. Available from: http://www.science-line.com/index/; http://www.ojafr.ir

[34] Ukpebor JE, Akpaja EO, Ukpebor EE, Egharevba O, Efedue E. Effect of the edible mushroom, Pleurotus tuberregium on the cyanide level and nutritional contents of rubber seed cake. Pak J Nutr. 2007;6(6):534-7. https://doi.org/10.3923/pjn.2007.534.537

[35] Babatunde GM, Pond WG. Nutritive value of Nigerian rubber seed (Hevea brasiliensis) meal and oil. IV. Effects of graded levels of rubber seed meal and oil on energy and nitrogen utilization by growing rats. Anim Feed Sci Technol. 1990;31(3-4):313-21. https://doi.org/10.1016/0377-8401(90)90136-V

[36] Narahari D, Kothandaraman P. Chemical composition and nutritional value of para-rubber seed and its products for chickens. Anim Feed Sci Technol. 1984;10(4):257-67. https://doi.org/10.1016/0377-8401(84)90021-X

[37] Oluodo LA, Huda N, Komilus CF. Potential utilization of rubber seed meal as feed and food. Int J Eng Technol. 2018;7(4.43):64-71. https://doi.org/10.14419/ijet.v7i4.43.25821

[38] Sharma BB, Saha RK, Saha H. Effects of feeding detoxified rubber seed meal on growth performance and haematological indices of Labeo rohita (Hamilton) fingerlings. Anim Feed Sci Technol. 2014;193:84-92.

https://doi.org/10.1016/j.anifeedsci.2014.03.008

[39] Liu J, Zhao L, Cai H, Zhao Z, Wu Y, Wen Z, et al. Antioxidant and anti-inflammatory properties of rubber seed oil in lipopolysaccharide-induced RAW 267.4 macrophages. Nutrients. 2022;14(1349). https://doi.org/10.3390/nu14071349

[40] Oyewusi PA, Akintayo ET, Olaofe O. The proximate and amino acid composition of defatted rubber seed meal. J Food Agric Environ. 2007;5(3&4):115-8.

[41] Akintayo ET, Bayer E. Characterisation and some possible uses of Plukenetia conophora and Adenopus breviflorus seeds and seed oils. Bioresour Technol. 2002;85(1):95-7. https://doi.org/10.1016/S0960-8524(02)00073-1

[42] Farr HM, Donkoh A, Boateng M, Mensah KB. Effect of variously-processed rubber seed meals on reproductive performance: The use of Sprague-Dawley laboratory rats as model for pigs. J Anim Sci Vet Med. 2019;4(5):167-72.

https://doi.org/10.31248/JASVM2019.144

[43] Tean B, Sath K, Samkol P, Ly J. Utilization by pigs of diets containing Cambodian rubber seed meal. Livest Res Rural Dev. 2002;14(1). Available from: http://www.cipav.org.co/lrrd/lrrd14/1/ly141.htm

[44] Lacey JM, Wilmore DW. Is glutamine a conditionally essential amino acid? Nutr Rev. 1990;48(8):297-309.

https://doi.org/10.1111/j.1753-4887.1990.tb02967.x

[45] Agbai CM, Olawuni IA, Ofoedu CE, Ibeabuchi CJ, Okpala COR, Shorstkii I, et al. Changes in anti-nutrient, phytochemical, and micronutrient contents of different processed rubber (Hevea brasiliensis) seed meals. PeerJ. 2021;9:e11327.

https://doi.org/10.7717/peerj.11327

[46] Putra NR, Abdul Aziz AH, Rizkiyah DN, Che Yunus MA, Alwi RS, Qomariyah L. Green extraction of valuable compounds from rubber seed trees: A path to sustainability. Appl Sci. 2023;13(24):13102. https://doi.org/10.3390/app132413102

[47] Adesina SA. Effect of processing on the proximate composition of sunflower (Helianthus annuus) seeds. Agro-Science. 2019;17(3). https://doi.org/10.4314/as.v17i3.5

[48] Agbai CM, Olawuni IA, Azeke AE, Nwokenkwo EC, Alagbaoso SO. Evaluation of proximate and functional properties of rubber (Hevea brasiliensis) seed meals. Afr J Food Sci. 2020;14(11):407-13. https://doi.org/10.5897/AJFS2020.2033

How to cite this paper

Assessing the Nutritional Value and Suitability of Processed Rubber (Hevea Brasiliensis) Seed Meal for Rabbit Feed: A Focus on Anti-nutrient Degradation and Processing Methods

How to cite this paper: Obanla R. Oyinlola, Imen Bousrih, Ojewumi E. Modupe, Farouk U. Mohammed, Akerele E. Mojoyinoluwa. (2025) Assessing the Nutritional Value and Suitability of Processed Rubber (Hevea Brasiliensis) Seed Meal for Rabbit Feed: A Focus on Anti-nutrient Degradation and Processing MethodsInternational Journal of Food Science and Agriculture, 9(1), 1-15.

DOI: http://dx.doi.org/10.26855/ijfsa.2025.03.001