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Nanomaterials and Nanotechnology Advances

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ArticleOpen Access http://dx.doi.org/10.26855/nna.2024.12.001

Application of CRISPR/Cas-based Biosensing Platforms in Molecular Diagnostics

Hua Zhang

Nantong University, Nantong 226019, Jiangsu, China.

*Corresponding author: Hua Zhang

Published: November 29, 2024

Abstract

The CRISPR/Cas system is widely used in genome editing. The CRISPR/Cas system can precisely cleave and recognize DNA and RNA sequences. In the process of recognizing a target sequence, the CRISPR/Cas system not only recognizes the target sequence and initiates target-cleaving activity, but also shows non-specific cleavage activity on single strands such as DNA and RNA molecules, and emits fluorescence. is appended. Group of reporter nucleic acid molecules to be added in the reaction system, the positions of target nucleic acid molecules can be observed by fluorescence signals, and multiple target molecules can be detected simultaneously due to the different cleavage priorities of Cas proteins.

Keyword

Molecular diagnostics; CRISPR/Cas; biosensing platform; application value

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Copyright

© 2024 by the author(s).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND) license, which permits non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited and is not modified or adapted.
https://creativecommons.org/licenses/by-nc-nd/4.0/

How to cite this paper

Application of CRISPR/Cas-based Biosensing Platforms in Molecular Diagnostics

How to cite this paper: Hua Zhang. (2024). Application of CRISPR/Cas-based Biosensing Platforms in Molecular Diagnostics. Nanomaterials and Nanotechnology Advances, 1(1), 1-4.

DOI: http://dx.doi.org/10.26855/nna.2024.12.001