Xuefei Xiao1,*, Tao Dong1, Zhaoping Li2
1Kaifeng Product Quality Inspection and Testing Center, Kaifeng, Henan, China.
2Kaifeng Food and Drug Center for Quality and Safety, Kaifeng, Henan, China.
*Corresponding author: Xuefei Xiao
Abstract
This study aims to compare the clinical application of the paper disk method and the Baird Parker plate method in the quantitative detection of Staphylococcus aureus in food. These two methods were used to quantitatively detect Staphylococcus aureus in 6 food samples, and the results were statistically analyzed. The research results showed that the detection rate of Staphylococcus aureus using the paper method was 31.67%, while the detection rate of the Baird Parker plate method was 20.42%. There is a significant difference in detection rates between the two methods, and this difference is statistically significant (P<0.05). Although the detection rate of the Baird Parker plate method is relatively low, the paper method, as a simple and highly valuable method for promotion, still has certain advantages. It should be noted that the detection cost of the paper method is relatively high, which may be a limiting factor it faces in practical applications. However, considering its simplicity of operation and high detection rate, the paper method is still a feasible choice, especially in some resource-limited environments. In addition, the research results provide valuable references for food safety monitoring of Staphylococcus aureus. However, further research is needed to validate the applicability of these methods in a wider range of food samples and to compare their differences with other commonly used methods. In addition, it is necessary to consider other factors such as sensitivity, specificity, and time efficiency of the detection to select the most suitable method for quantitative detection of Staphylococcus aureus.
References
[1] Zhao Ping, Chen Fei, & Zhou Jiahong. Evaluation of SYBR Green qPCR method for rapid detection of Staphylococcus aureus in peanut products [J]. Journal of Nanjing Normal University: Engineering & Technology Edition, 2023, 23(2): 69-76.
[2] Bai X B, Suo J, Cao C Y, et al. Analysis of toxin coding genes and drug susceptibility of Staphylococcus aureus derived from raw food vegetables [J]. Food Science, 2023, 44(14): 94-99.
[3] Zhou Y, Wu XW, Hu Yushan, et al. Contamination of Staphylococcus aureus in ready-to-eat foods in Guangzhou from 2009 to 2018 and bacterial type characteristics [J]. Chinese Journal of Food Hygiene, 2021, 33 (4): 7. DOI: 10.13590 / j.carol carroll JFH 2021.04.008.
[4] Qin J, Wang L F, Wei S Y, et al. Verification results and analysis of quantitative detection ability of Staphylococcus aureus in FAPAS milk powder [J]. Food Safety Guide, 2021, issue 26, pp. 82-83.
[5] Ma Ran, Lei Liubing, & Li Fajun. Verification results and analysis of detection ability of Staphylococcus aureus in cosmetics [J]. Quality Safety and Inspection and Testing, 2021, 31(5): 4.
[6] Cui Jie, Huang Zhuliang, Yan Zhuoyan, et al. Establishment of a multiplex fluorescence quantitative PCR method for detection of three foodborne pathogens in ready-to-eat food in bulk [J]. Food Science and Technology, 2023.
[7] Liu Shuang, Huang Xiaoying, Su Yahang, et al. Inhibitory mechanism of lactic acid bacteria extracts isolated from traditional fermented food against methicillin-resistant Staphylococcus aureus [J]. Journal of Food Safety and Quality Inspection, 2023, 14(1): 8.
[8] Wu L W, Yang Z S, Tang X Z, et al. Investigation of Staphylococcus aureus contamination in food in Yunnan Province from 2019 to 2021 [J]. Chinese Journal of Frontier Health and Quarantine, 2023(005): 046.
[9] Yuan Shuhong, Rui Hongfei, Liu Qing, et al. Analysis of Staphylococcus aureus contamination and enterotoxin and drug resistance in milk tea in Lin'an District of Hangzhou [J]. Chinese Journal of Health Inspection, 2023.
[10] Li X Z, LI Z, & Liu D. Identification and analysis of Staphylococcus aureus in pharmaceuticals by four microbial identification methods [J]. Capital Food and Medicine, 2023.
How to cite this paper
Analysis of Staphylococcus Aureus in Food by Paper Method and Baird-Parker Plate Method
How to cite this paper: Xuefei Xiao, Tao Dong, Zhaoping Li. (2023) Analysis of Staphylococcus Aureus in Food by Paper Method and Baird-Parker Plate Method. International Journal of Food Science and Agriculture, 7(4), 506-510.
DOI: https://dx.doi.org/10.26855/ijfsa.2023.12.014