Caihong Hao, Li Li*, Guizhi Tong, Botao Jiang, Jiansheng Zhang, Yan Zhang, Zhenhua Jin, Likun Wang, Haiyang You, Jiahou Wang, Wenkai Guo, Ping Li
Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences, Qiqihar, Heilongjiang, China.
*Corresponding author: Li Li
Abstract
With the development of assisted reproductive technology, embryo freezing has become an inseparable part of its development. Cryopreservation of donkey embryos has become a recognized technique in donkey-assisted reproduction. However, it has not been determined which stage of cryopreservation of donkey embryos before transplantation is the most effective. During programmed freezing, cells gradually dehydrate in a balanced manner, with different concentrations of molecules and ions in ice crystals and nonice crystals. Vitrified freezing uses a high concentration of protective agent for rapid cooling, allowing all liquids to harden without forming ice crystals. The distribution of molecules and ions in a solid substance after vitrification is the same as that of the original liquid, so this solid substance can also be considered a very viscous and low-temperature liquid. Programmed slow freezing is a relatively mature freezing technology, widely used in the field of donkey-assisted reproduction. Currently, vitrification is a new freezing method, which is simpler and more effective than traditional slow freezing. It has certain advantages in terms of recovery rate, blastocyst formation rate, and blastocyst hatching rate. In addition, with the application of vitrified donkey embryo freezing technology, the laboratory workload is reduced, the cost is reduced, the number of attempts to conceive per ovulation is maximized, and the practicability is improved.
References
[1] Shkedi-Rafid, S. (2011). Egg freezing for age-related fertility decline: preventive medicine or a further medicalization of reproduction? Analyzing the new Israeli policy. Fertility and Sterility, 96(2), 291-294.
[2] Smith, G. D., Serafini, P. C., Fioravanti, J., Yadid, I., Coslovsky, M., Hassun, P., Motta, E. L. (2010). Prospective randomized comparison of human oocyte cryopreservation with slow-rate freezing or vitrification. FertilSteril, 94(6), 2088-2095.
[3] Tanaka, M. (2005). H1FOO is coupled to the initiation of oocytic growth. Biology of reproduction, 72.
[4] The practice of Committees of the American Society for Reproductive Medicine and the Society for Assisted Reproductive. (2013). Mature oocyte cryopreservation: a guideline. Fertility and Sterility, 99(1). 37-43.
[5] Trokoudes, K. M., Pavlides, C., & Zhang, X. (2011). Comparison outcome of fresh and vitrified donor oocytes in an egg-sharing donation program. FertilSteril, 95(6), 1996-2000.
[6] Jaroudi S, Sengupta S. (2007). DNA repair in mammalian embryos. Mutat Res, 2007, 635(1):53-77.
[7] Cantone I, Fisher AG. Epigenetic programming and reprogramming during development. Nat StructMolBiol, 2013, 20(3):282-289.
[8] Hales BF, Grenier L, Lalancette C, Robaire B. (2011). Epigenetic programming: from gametes to blastocyst. Birth Defects Res A ClinMolTeratol, 2011, 91(8):652-665.
[9] Dorado, J.; Bottrel, M.; Ortiz, I.; Díaz-Jiménez, M.; Pereira, B.; Consuegra, C.; Carrasco, J.J.; Gómez-Arrones, V.; Domingo, A.; Hidalgo, M. (2020). Factors Affecting Embryo Recovery Rate, Quality, and Diameter in Andalusian Donkey Jennies. Animals, 2020, 10, 1967. https://doi.org/10.3390/ani10111967.
[10] Tingjin Chang, Mei Li, Xiaoya An, Fuxia Bai, Fuwen Wang, Jie Yu, Chuzhao Lei & Ruihua Dang. (2023). Association analysis of IGF2 gene polymorphisms with growth traits of Dezhou donkey, Animal Biotechnology, 34:4, 1143-1153.
DOI: 10.1080/10495398.2021.2013860.
How to cite this paper
Study on the Technology of Vitrified Frozen Donkey Embryos
How to cite this paper: Caihong Hao, Li Li, Guizhi Tong, Botao Jiang, Jiansheng Zhang, Yan Zhang, Zhenhua Jin, Likun Wang, Haiyang You, Jiahou Wang, Wenkai Guo, Ping Li. (2023) Study on the Technology of Vitrified Frozen Donkey Embryos. International Journal of Food Science and Agriculture, 7(3), 386-389.
DOI: http://dx.doi.org/10.26855/ijfsa.2023.09.007