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International Journal of Clinical and Experimental Medicine Research

ISSN Online: 2575-7970 ISSN Print: 2575-7989 CODEN: IJCEMH
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ArticleOpen Access http://dx.doi.org/10.26855/ijcemr.2023.04.008

Effect of Hydrogen on the Drp 1-dependent Mitochondria after Brain I/R

Rong Hu*, Yong Zhu, Juan Liu, Ci Jiang, Hui Huang, Youyuan Chen, Dandan Chen, Yishan Xiong

Medical College, Hunan University of Medicine, Huaihua, Hunan, China.

*Corresponding author: Rong Hu

Published: June 2,2023

Abstract

Objective: To evaluate the effect of hydrogen on the apoptotic pathway of Drp 1-dependent mitochondria division after brain I/R. Methods: 60 clean-grade male SD rats (200-220g) were divided into 3 groups by random numbers, blank (n=20); I/R (n=20) and I /R + H2 (n=20). Rat brain I/R injury model was made by the line-bolt method. Group I/R + H2 was injected with hydrogen intraperitoneally 1h and 6h, respectively. Groups I/R and I/R + H2 were severed under anesthesia after successful surgery, and the cerebral infarction area was observed by TTC staining. SD rats were killed at 6, 12, and 24h after surgery, and the expression of mitochondrial motility-related protein 1 (Drp 1) was determined in the brain tissue by Western blot. Results: The TCC staining showed that the treatment with the I/R rats reduced the infarction area of cerebral ischemia and reperfusion. Drp 1 expression was up-regulated in I/R and I/R + H2 groups (P <0.05); Drp 1 expression was downregulated in I/R + H2 groups compared with I/R groups (P <0.05). Conclusion: The mechanism by which hydrogen gas improves the mitochondrial function in I/R rats may be related to its promotion of mitochondrial fusion and the inhibition of mitochondrial fission.

Keywords

Hydrogen, I/R, Drp 1, and mitochondria

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How to cite this paper

Effect of Hydrogen on the Drp 1-dependent Mitochondria after Brain I/R

How to cite this paper: Rong Hu, Yong Zhu, Juan Liu, Ci Jiang, Hui Huang, Youyuan Chen, Dandan Chen, Yishan Xiong. (2023) Effect of Hydrogen on the Drp 1-dependent Mitochondria after Brain I/R. International Journal of Clinical and Experimental Medicine Research7(2), 144-148.

DOI: http://dx.doi.org/10.26855/ijcemr.2023.04.008