International Journal of Food Science and Agriculture

ISSN Print: 2578-3467 Downloads: 161363 Total View: 2557214
Frequency: quarterly ISSN Online: 2578-3475 CODEN: IJFSJ3
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Article http://dx.doi.org/10.26855/ijfsa.2023.03.024

Establishment and Application of the Dual PCR Detection Method for Swine Respiratory and Reproductive Syndrome Virus (PRRSV) and Swine Pseudorabies Virus (PRV)

Zhiliang Li

College of Veterinary Medicine, Northwest A&F University, Xianyang, Shaanxi, China.

*Corresponding author: Zhiliang Li

Published: May 6,2023

Abstract

In recent years, there are more and more mixed infections or secondary infections caused by porcine reproductive respiratory syndrome virus and porcine rabies virus, which complicate the diagnosis, treatment and prevention for the rapid diagnosis of these two pathogens. Specific primer pairs were developed by sequence comparison and related literature based on the gene sequence related to the PRRSV and PRV viruses published on the official website of the National Center for Bioinformatics (NCBI). Using nucleic acids PRRSV and PRV as models, dual PCR assays were developed to rapidly identify PRRSV and PRV. The experimental results show that this method can obtain specific target fragments of 401 bp and 261bp for amplified PRV and PRRSV and negative for amplified PEDV, CSFV and ASFV nucleic acids. Using the sensitivity experiments, the detection limits for PRV and PRRSV were 0.12 pg and 0.045 pg, respectively. The dual detection method established for PRRSV and PRV has a strong specificity and a good sensitivity, which can be used for the identification of these two pathogens.

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How to cite this paper

Establishment and Application of the Dual PCR Detection Method for Swine Respiratory and Reproductive Syndrome Virus (PRRSV) and Swine Pseudorabies Virus (PRV)

How to cite this paper:  Zhiliang Li. (2023) Establishment and Application of the Dual PCR Detection Method for Swine Respiratory and Reproductive Syndrome Virus (PRRSV) and Swine Pseudorabies Virus (PRV). International Journal of Food Science and Agriculture7(1), 172-175.

DOI: http://dx.doi.org/10.26855/ijfsa.2023.03.024