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International Journal of Food Science and Agriculture

ISSN Print: 2578-3467 Downloads: 160031 Total View: 2550920
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Article http://dx.doi.org/10.26855/ijfsa.2022.09.017

Determination of Aflatoxins in Different Varieties of Chillies Collected from Lahore, Pakistan

Naseem Zahra1,*, Nayyab Naeem2, Aaliya Iqbal Butt2, Muhammad Khalid Saeed1, Jannat Akram2, Esha Gulzar1

1Food and Biotechnology Research Centre, PCSIR Laboratories Complex, Ferozepur road, Lahore-54600, Pakistan. 

2Govt.  Postgraduate College for Women Gulberg, Lahore, Pakistan.

*Corresponding author: Naseem Zahra

Published: September 28,2022

Abstract

Aflatoxin is a potent carcinogenic, curious metabolite mainly produced by many fungal species such as A. flavus and A. parasiticus. AFs are very toxic food contaminants that can acutely damage human health. There are four main groups of aflatoxins which include B1, B2, G1, and G2. A survey of red chili for aflatoxin contamination was performed in different varieties of chilies including Capsicum annum, L. Piper nigrum, L. C. frutescence, and fresh green chilies. In the present study detection of aflatoxin contamination in red chilies was analyzed through TLC (Thin Layer Chromatographic) technique. A total of 20 various red chili samples were collected from different local regions of Lahore, Pakistan. The occurrence of aflatoxin B1 was detected in 9 samples of chilies. In the case of red powdered chilies, 2 samples were contaminated with aflatoxin B1 ranging between 11.88ppb-11.89ppb. These two contaminated samples are unfit for human health and beyond the permissible limits. In the case of red round chilies, 3 samples were contaminated with aflatoxin B1 and its concentration ranged between 4.68ppb-13.37ppb. These two contaminated samples are unfit for consumption and outside the satisfactory limits. One contaminated sample is fit and below the permissible limit. While in P. nigrum four samples were contaminated with aflatoxin B1 ranging between 2.21ppb-13.47ppb. In which two contaminated samples were unfit for human health and outside the satisfactory limits. The other two contaminated samples were fit for consumption and below the satisfactory limits. In fresh green chilies, all samples were found to be free from contamination. While other types of aflatoxins B2, G1, and G2 were absent in all the samples. The relative percentage of aflatoxin contamination in C. annum (red chilli) was 40%, C. frutescence (round red chilli) was 60%, P. nigrum (black pepper) was 80% and fresh green chili was found to be free from contamination. The supply of red chilies in Lahore is extremely dangerous to human health because when exceeded beyond permissible level, aflatoxin can cause cancer. The main reason for aflatoxin contamination in chilies samples is the lack of implementation of qualitative methods.

References

[1] Zeringue, H. J., & Shih, B. Y. (1998). Extraction and Separation of the Bright-Greenish-Yellow Fluorescent Material from Aflatoxigenic Aspergillus spp. Infected Cotton Lint by HPLC-UV/FL. Journal of Agricultural and Food Chemistry, 46(3), 1071–1075. https://doi.org/10.1021/JF9707391.  

[2] Curto, R., Pellicano, T., Vilasi, F., Munafo, P., Chemistry, G. D.-F., & 2004, U. (2000). Ochratoxin A occurrence in experimental wines in relationship with different pesticide treatments on grapes. Elsevier. https://www.sciencedirect.com/science/article/pii/S0308814603001687.

[3] Goto, T., Wicklow, D. T., & Ito, Y. (1996). Aflatoxin and cyclopiazonic acid production by a sclerotium-producing Aspergillus tamarii strain. Applied and Environmental Microbiology, 62(11), 4036-4038. https://doi.org/10.1128/AEM.62.11.4036-4038.1996.

[4] Klich, M. A., Mullaney, E. J., Daly, C. B., & Cary, J. W. (2000). Molecular and physiological aspects of aflatoxin and sterigmatocystin biosynthesis by Aspergillus tamarii and A. ochraceoroseus. Applied Microbiology and Biotechnology, 53(5), 605-609. 

[5] Paterson, R. R. M., & Lima, N. (2010). Toxicology of mycotoxins. EXS, 100, 31-63. https://doi.org/10.1007/978-3-7643-8338-1_2.

[6] IARC. (2002). Some traditional herbal medicines, some mycotoxins, naphthalene and styrene. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans/World Health Organization, International Agency for Research on Cancer, 82, 1-556. 

[7] Madalı, B., & Ayaz, A. (2017). Aflatoxin M1 in Dairy Products: Exposure and Health Risks. Hacettepe Üniversitesi Sağlık Bilimleri Fakültesi Dergisi, 4(1), 1-14. https://doi.org/10.21020/HUSBFD.296196.

[8] Arumugam, V., Ooi, K. B., & Fong, T. C. (2008). TQM practices and quality management performance: An investigation of their relationship using data from ISO 9001:2000 firms in Malaysia. TQM Journal, 20(6), 636-650. 

[9] Yusof, S. M., & Aspinwall, E. (2001). Case studies on the implementation of TQM in the UK automotive SMEs. International Journal of Quality and Reliability Management, 18(7), 722–743. https://doi.org/10.1108/02656710110396058/FULL/HTML.

[10] Arim, R. H. (1995). Present status of the aflatoxin situation in the philippines. Food Additives and Contaminants, 12(3), 291–296. https://doi.org/10.1080/02652039509374306.

[11] Njapau, H., Muzungaile, E. M., & Changa, R. C. (1998). The effect of village processing techniques on the content of aflatoxins in corn and peanuts in Zambia. Journal of the Science of Food and Agriculture, 76(3), 450-456.

[12] Zahra, N., Muhammad K. S., Muhammad A., Fatima S. and Areesha S. (2022). Detoxification of Aflatoxins in Food Commodities by Various Methods, Chemical Science & Engineering Research, 4(9), 9-13.

[13] Sakin, F., Tekeli, İ., Yipel, M., Control, C. K.-F., & 2018, U. (2010). Occurrence and health risk assessment of aflatoxins and ochratoxin a in Sürk, a Turkish dairy food, as studied by HPLC. Elsevier. https://www.sciencedirect.com/science/article/ pii/S0956713518301154.

[14] Summia, K., Yasmeen, R., & Zahra, N. (2021). Detection of aflatoxins b1 from layer and broiler feed samples collected from different cities of Punjab. Pakistan. J. Anim. Health Prod, 9(4), 435-442. 

[15] Elameen, M. I. H. (2014). Occurrence and control of seed borne fungi of four food crops in Sudan (Doctoral dissertation, Sudan University of Science and Technology).

[16] Eskola, M., Kos, G., Elliott, C. T., Hajšlová, J., & Krska, R. (2020). Worldwide contamination of food-crops with mycotoxins: Validity of the widely cited ‘FAO estimate’ of 25%. Taylor & Francis, 60(16), 2773-2789. https://doi.org/10.1080/10408398.2019.1658570.

[17] Abrar, M., Anjum, F., Zahoor, T., Nutr, H. N.-P. J., & 2009, undefined. (2009). Effect of storage period and irradiation doses on red chillies. Citeseer, 8(8), 1287-1291. 

[18] Set, E., & Erkmen, O. (2010). The aflatoxin contamination of ground red pepper and pistachio nuts sold in Turkey. Food and Chemical Toxicology, 48(8–9), 2532–2537. https://doi.org/10.1016/j.fct.2010.06.027. 

[19] Zahra, N., Khan, M., Mehmood, Z., Saeed, M. K., Kalim, I., Ahmad, I., & Malik, K. A. (2018). Determination of aflatoxins in spices and dried fruits. Journal of Scientific Research, 10(3), 315-321. 

[20] Zahra, N., Jamil, N., Ahmad, S. R., Munir, S., Saeed, M. K., & Kalim, I. (2017). Comparative analysis of brown and white rice samples for the presence of Aflatoxin B1 contamination and effect of environment temperature on Aflatoxin concentration during storage. Transylvanian Review, 25, 4507-4515.

[21] Zahra, N., Firdous, S., Ejaz, N., & Hina, S. (2012). Detection of aflatoxins in various samples of red chilli. Biological Sciences-PJSIR, 55(1), 27-29.

[22] Iqbal, S. Z., Paterson, R. R. M., Bhatti, I. A., & Asi, M. R. (2011). Comparing aflatoxin contamination in chilies from Punjab, Pakistan produced in summer and winter. Mycotoxin research, 27(2), 75-80.

[23] Commission EC. (2002). Commission Regulation (EC) No. 472/2002 of 12 March 2002. Brussels,, Belgium: L, 75: 18-20. 

[24] Manual of Food Laws in Pakistan. (2004). Pure food rules, 1965. In: Manual of Food Law of Pakistan, 2004 edn. Irfan Law Book House, Lahore, pp. 83-161.

How to cite this paper

Determination of Aflatoxins in Different Varieties of Chillies Collected from Lahore, Pakistan

How to cite this paper: Naseem Zahra, Nayyab Naeem, Aaliya Iqbal Butt, Muhammad Khalid Saeed, Jannat Akram, Esha Gulzar. (2022) Determination of Aflatoxins in Different Varieties of Chillies Collected from Lahore, Pakistan. International Journal of Food Science and Agriculture, 6(3), 349-354.

DOI: http://dx.doi.org/10.26855/ijfsa.2022.09.017

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